Tli DNA polymerase

Tli DNA polymerase is a thermal stability of the molecular weight of about 90 kDa, the enzyme in 74 ℃ copy DNA, in the half-life of 100 ℃ for two hours. The enzyme in the conditions for the existence of magnesium can be catalytic nucleotide along the 5'-3 'direction in polymerization, a double-stranded DNA, the enzyme also has 3'-5' correction activity, can improve the security nucleotide incorporation I degrees. Tli DNA polymerase Thermococus litoralis is derived from the DNA polymerase I containing the gene fragments in E. coli E. Coli in Cloning and Expression of the reorganization. Tli DNA polymerase is recommended for high-fidelity PCR reaction and high temperature conditions primer extension of reaction. Heat-stable DNA polymerase 10 × reaction buffer: 500mM KCl, 100mM Tris-HCl (pH9.0, 25 ℃), 1.0% Triton ® X-100. Buffer optimizing the use of 0.2 mM dNTP. Magnesia: contains 25 mM MgCl2 solution. Features ● stability: In the half-life of 100 ℃ for two hours ● flexible: System provides a non-MgCl2 the 10 × reaction buffer and a separate 25 mM MgCl2 solution, for the different conditions for the optimization of reaction. Apply ● high-fidelity PCR Storage conditions: -20 ℃ save storage buffer: 10 mM Tris-HCl (pH 7.4,25 ℃), 100mM KCl, 0.1mM EDTA, 1mM DTT and 0.1% Triton ® X-100, 50% glycerol. Units definition: In the 74 ℃ conditions, within 30 minutes by 10 nmol dNTP response to the introduction of a TCA insoluble material necessary for a unit of volume. Conditions are: 50 mM Tris-HCl (pH 9.0,25 ℃), 50mM NaCl, 10mM MgCl2, 200mM dNTP (with no tags and tags [3 H] dTTP), 11mg activation of calf thymus DNA, for the final reaction volume 50 ml. Quality Control Testing: active, DNA endonuclease / lack of carved.